Coding
Part:BBa_K2881004:Design
Designed by: Zhijian Li Group: iGEM18_UC_San_Diego (2018-10-10)
hMBD-eGFP-avi-His
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1027
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Compared to its analog part BBa_K2881003 (mMBD-eGFP-avi-His), this part consists of human MBD domain, which has a higher affinity toward methylated CpG site. However, it is relatively insoluble when expressed in E.coli BL-21, giving it a significantly lower yield. Compared to mMBD construct it is more suitable for small scale synthesis. Further improvement of the part includes other purification strategies such as GST fusion.
Source
gBlocks of different parts were purchased from IDT DNA and then assembled into vectors.