Part:BBa_K2881004:Design

hMBD-eGFP-avi-His

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1027
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Compared to its analog part BBa_K2881003 (mMBD-eGFP-avi-His), this part consists of human MBD domain, which has a higher affinity toward methylated CpG site. However, it is relatively insoluble when expressed in E.coli BL-21, giving it a significantly lower yield. Compared to mMBD construct it is more suitable for small scale synthesis. Further improvement of the part includes other purification strategies such as GST fusion.

Source

gBlocks of different parts were purchased from IDT DNA and then assembled into vectors.